【摘要】 目的探讨血管内皮生长因子(VEGF)在蛛网膜下腔出血(SAH)后早期脑损伤中的分子机制。方法采用非开颅血管内穿线法制备大鼠SAH模型,108只大鼠按随机数字表法分为正常对照组(n=36)、假手术组(n=36)、模型组(n=36),术后行神经功能评分,分别在伤后12、24、48h3个时相点取脑组织标本,HE染色及电镜观察右侧海马CA1区组织学变化,采用免疫组织化学法、免疫印迹法检测VEGF的表达,TUNEL法检测凋亡细胞表达。结果与对照组比较,模型组大鼠神经功能评分均显著降低(P<0.05),神经细胞内细胞器、轴索及毛细血管等超微结构明显受损,存活数目明显降低。模型组(12、24、48h)大鼠VEGF阳性反应均有不同程度增强(P<0.05),随出血时间延长(12、24、48h),凋亡细胞增多(P<0.05),并于48h达高峰。SAH12～48h海马区VEGF与TUNEL表达呈正相关(r=0.847,P<0.01)。结论 SAH后VEGF高表达可促进神经细胞凋亡,参与SAH后早期脑损伤的病理进程。更多还原

【Abstract】 Objective To investigate the molecular mechanism of vascular endothelial growth factor(VEGF) for early brain injury after subarachnoid hemorrhage(SAH).Methods A total of 90 male Sprague-Dawley rats were randomly divided into 3 groups,control group(n=30),sham operation group(n=30),and SAH group(n=30).SAH model was established by endovascular perforation without opening cranium,and then neurological score was determined.At different time points(12,24,and 48 h) after operation,the tissues of hippocampus CA1 area were sampled.The histopathological changes of cerebral tissue were observed by HE staining,and ultrastructural changes by electron microscopy.The expression of VEGF were determined by immunohistochemical staining and Western blot analysis.The apoptosis of nerve cell were detected by TUNEL.Results In SAH group,the neurological function scores were significantly lower(P<0.05).The axons and capillaries in SAH group were obviously damaged,the living neurons were much lesser than in sham group,the VEGF level was significantly enhanced(P<0.05),and a lot of apoptosis cells were observed and their number reached a peak at 48 h(P<0.05).The expression of VEGF was positively correlated with the TUNEL positive cells after SAH(r=0.847,P<0.01).Conclusion VEGF contributes to early brain injury,and the increased expression of VEGF in hippocampal CA1 may induce neuronal apoptosis following SAH.更多还原