【摘要】 以切花百合(Lilium)品种‘黄天霸’(‘Manissa’)花器官为外植体诱导体细胞胚胎发生与植株再生。结果表明,不同花器官、不同激素配比对愈伤组织形成均具有显著影响。花丝为最佳外植体,激素对愈伤组织诱导的影响效应为NAA>6-BA>2,4-D,最适培养基为MS+1.0 mg.L-1NAA+0.2 mg.L-16-BA;激素诱导体细胞胚胎发生的影响效应为2,4-D>KT>6-BA,最佳培养基配方为MS+1.0 mg.L-12,4-D+0.2 mg.L-1KT+1.0 mg.L-16-BA;MS培养基添加IBA可促进体细胞胚萌发成苗,体细胞胚芽成苗的最佳培养基为MS+0.2 mg.L-16-BA+1.0 mg.L-1IBA。更多还原

【Abstract】 Studies on somatic embryogenesis and plant regeneration of Lilium ’Manissa’ from the different parts of floral organ explants were performed.The results showed that filament was the appropriate explants for embryogenic callus induction.The effects of hormones on callus formation was NAA>6-BA>2,4-D.The suitable medium for callus induction was MS+1.0 mg·L-1 NAA+0.2 mg·L-1 6-BA.The effects of hormones on somatic embryogenesis from embryogenic callus was 2,4-D>KT>6-BA.Embryogenic calluses were inducted on medium MS+1.0 mg·L-1 2,4-D+0.2 mg·L-1 KT+1.0 mg·L-1 6-BA.IBA could promote somatic embryo germination.The optimum embryoid geminating medium was MS+0.2 mg·L-1 6-BA+1.0 mg·L-1 IBA.更多还原