【摘要】 以中小叶茶树良种舒茶早和云南大叶种73-11为材料,于4℃低温处理4 d后,采用AFLP技术筛选出冷诱导下茶树差异表达基因并进行分析.结果显示,实验共获得41个差异片段(TDFs),其中舒茶早产生19条差异条带,73-11产生38条差异条带,两品种共有条带16条.所获得的差异片段按功能可划分为4类,即信号传导蛋白、转录因子、基础代谢相关蛋白、抗逆蛋白质,此外还有一些假设蛋白质以及未知蛋白.利用qRT-PCR对Kh1、Kh3和Kh11差异片段进行验证,结果显示这3个片段均被低温诱导,表达量上升.研究表明,茶树在低温胁迫下的逆境反应非常复杂,涉及多种代谢过程中的众多基因,而且大叶种比中小叶种对低温反应更为敏感.更多还原

【Abstract】 The cold regulated genes were screened from both Yunnan large-leaved cv.’73-11’ and small-medium leaved cv.’Shuchazao’under low temperature stress for 4 days using cDNA-AFLP.The results showed that 41 Transcript-Derived Fragments(TDFs) were obtained,including 38 TDFs from cv.’73-11’,19 TDFs from cv.’Shuchazao’,and 16 TDFs shared by the two varieties.According to blasting results,these TDFs were classified into four groups:signal transduction genes,transcription factors,primary metabolism genes and proteins related to stress.In addition,there were some genes for predicted protein and unknown protein.The results of qRT-PCR for Kh1,kh3 and kh11,showed that expression of these TDFs increased obviously under low temperature stress.It had been shown that response of tea plant to low-temperature stress is very complex with many genes of several metabolism involved,and cv.’73-11’ is more sensitive to the low temperature stress than cv.’Shuchazao’.更多还原