节点文献
紫贻贝多烯脂肪酸乙酯及牛磺酸的提取与分析
Extraction and Analysis of Polyenoic Fatty Acyl Ethyl Esters and Taurine in Mytilus edulis
【摘要】 为综合开发利用低价值贝类中的功能保健品成分,利用索氏/超声提取、尿素包合法提取了紫贻贝中的多烯脂肪酸乙酯,对提油后的残渣利用热水提取、离子交换层析富集的方法提取了其中的牛磺酸,利用高效液相色谱-二极管阵列检测器(HPLC-DAD)分析测定了产品中二十碳五烯酸乙酯(EPA-E)、二十二碳六烯酸乙酯(DHA-E)的含量,利用薄层色谱法、吸光光度法定性定量测定了产品中的牛磺酸,结果显示紫贻贝中含有相当量的多烯脂肪酸乙酯及牛磺酸成分,有望作为高价值功能保健食品的来源.
【Abstract】 For comprehensive utilization of the functional health products from the low-value shells,the polyunsaturated fatty acyl ethyl esters in Mytilus edulis were extracted by Soxhlet/supersonic extraction and urea complexation,and taurine in the residue after the fatty extraction was extracted by hot water digestion and ion-exchange chromatography.The contents of eicosapentaenoic acyl ether ester(EPA-E) and docosahexaenoic acyl ethyl esters(DHA-E) in the product were analyzed by HPLC-DAD.By thin layer chromatography and spectrophotometry,taurine in the crude product was qualitatively and quantitatively analyzed,respectively.The results show that the Mytilus edulis contains rich EPA,DHA and taurine and it is an expectable resource of high-value functional health food.
【Key words】 Mytilus edulis; EPA-E; DHA-E; taurine; extraction; analysis;
- 【文献出处】 大连交通大学学报 ,Journal of Dalian Jiaotong University , 编辑部邮箱 ,2011年02期
- 【分类号】TS254.1
- 【被引频次】3
- 【下载频次】217