【摘要】 目的:检测核转录因子(NF-κB)在子宫内膜异位症(EM)患者异位内膜及在位内膜组织中的表达,以及其血清中肿瘤坏死因子-α(TNF-α)的变化,探讨NF-κB与TNF-α在EM发病机制中的作用。方法:采用酶联免疫吸附试验(ELISA)测定TNF-α的表达,免疫组化SP法检测NF-κB的表达情况。结果:子宫内膜异位症(EM)组血清TNF-α含量显著高于对照组(P<0.01),且表达量随病情加重有上升趋势(P<0.05)。EM患者中异位内膜组NF-κB表达强度显著高于在位组及对照组(P<0.01)。结论:NF-κB与TNF-α在EM患者异位内膜中的异常高表达与EM的发病有密切关系。更多还原

【Abstract】 Objective: The expression of NF-κB in the tissue of endometria and tumor necrosis factor-α in serum in endometriosis patients were measured to investigate the relationship between NF-κB,TNF-α and endometriosis.Methods: Enzyme linked immunosorbent assay method was applied to detect the the expression of TNF-α.The expression of NF-κB was detected by immunohistochemistry method.Results: The TNF-α serum level of endometriosis patients was significantly higher compared to the normal control(P < 0.01).The expression of NF-κB was major located in epithelioglandular kytoplasm of trusion-endometriosis,and the expression of NF-κB during progestational stage was higher than that in proliferative stage(P < 0.05).Conclusion: The abnormal expression of NF-κB and TNF-α were related to endometriosis.更多还原