【摘要】 水溶性氧化还原介体可加速偶氮染料厌氧生物脱色,但会随出水而流失,造成二次污染.将醌还原菌群与非水溶性介体——蒽醌共固定化,考察了其对偶氮染料生物脱色的影响.克隆和RIS片段测序结果表明,醌还原菌群主要包括乳杆菌科(Lactobacillaceae)、肠杆菌科(Enterobacteriaceae)和拟杆菌科(Bacteroidaceae);共固定化醌还原菌群与蒽醌对10种不同结构偶氮染料的生物脱色速率提高1.3～2.1倍;共固定化菌球经10次循环使用后,对活性艳红KE-3B脱色率仍保持在85%以上.更多还原

【Abstract】 The decolorization rate of azo dyes under anaerobic conditions can be improved by the addition of water-soluble redox mediators.However,the continuous dosing and discharge of water-soluble redox mediators,such as biologically recalcitrant compounds,will result in the secondary contamination.The accelerating effect of co-immobilization of quinone-reducing consortium and insoluble anthraquinone in the biodecolorization of azo dyes was investigated for the first time.The cloning and sequencing analysis of ribosomal intergenic spacer (RIS) genes show that quinone-reducing community is predominate by Lactobacillaceae,Enterobacteriaceae!aod Bacteroidaceae;the decolorization rates of a broad range of azo dyes are greatly improved by co-immobilized beads as 1.3-2.1-fold increases;aftes ten repeated experiments,the decolorization rate of Active Red KE-3B by co-immobilized beads retains over 85%.更多还原