【摘要】 目的研究白介素-1β(IL-1β)对人胚肺成纤维细胞(FB)向肺肌成纤维细胞转化的影响及香菇多糖(LNT)的作用。方法体外培养人胚肺FB,用不同浓度IL-1β和LNT作用于细胞后,采用CCK-8法检测细胞的增殖情况,免疫细胞化学染色检测α-平滑肌肌动蛋白(α-SMA),RT-PCR检测纤维连接蛋白(FN)、Ⅰ型胶原蛋白(ColⅠ)和α-SAM mRNA的相对表达量。结果①IL-1β组细胞增殖的吸光度、α-SMA蛋白及FN、ColⅠ和α-SAMmRNA表达均高于对照组(P<0.01),且随着IL-1β浓度的增加,表达逐渐增高。②LNT呈浓度依赖性抑制IL-1β诱导的细胞增殖及α-SMA蛋白、FN、ColⅠ和α-SAM mRNA表达(P<0.01)。结论 LNT可抑制IL-1β诱导人胚肺FB增殖和肌成纤维细胞转化及细胞外基质积聚。[中国当代儿科杂志,2011,13(4):331-335]更多还原

【Abstract】 Objective To study the effects of interleukin-1β(IL-1β) on transdifferentiation of human embryonic lung fibroblasts to myofibroblasts and the effects of lentinan on the transdifferentiation.Methods The human embryonic lung fibroblasts were cultured in vitro,and fibroblasts were treated with different concentrations of IL-1β and lentinan.The proliferation activity of the human embryonic lung fibroblasts was evaluated by the Cell Counting Kit-8(CCK-8).The expression of α-smooth muscle actin(α-SMA) protein was measured by immunocytochemistry.The levels of fibronectin(FN),typeⅠcollagen(ColⅠ) and α-SMA mRNA were detected by RT-PCR.Results Compared with the untreated control group,the absorbance value of cell proliferation,α-SMA protein levels,FN,ColⅠand α-SMA mRNA expression were significantly up-regulated after different concentrations of IL-1β(0.1,1,10 ng/mL) treatment for 48 hrs(P<0.01).Lentinan treatment inhibited up-regulation of the cell proliferation activity,α-SMA protein levels,FN,ColⅠand α-SMA mRNA expression induced by IL-1β in a dose-independent manner(P<0.01).Conclusions Lentinan can suppress human embryonic lung fibroblast proliferation,fibroblast-myofibroblast transdifferentiation and extra cellular matrix synthesis induced by IL-1β.更多还原