【摘要】 以吉大豆1号、2号等5个大豆品种为材料,胚尖为外植体,研究不同浓度6-BA对不同基因型胚尖丛生芽诱芽率和再生率的影响,筛选得到适合于胚尖再生系统的基因型吉大豆2号,并以吉大豆2号为材料,研究侵染阶段加入脯氨酸和硝酸银对胚尖褐化率的影响,将获得的再生植株进行生根试验。结果表明:共培养基中添加3.0 mg.L-1脯氨酸和3.0 mg.L-1硝酸银能显著抑制褐化率,蔗糖配合较高浓度的IBA能更好地促进生根。更多还原

【Abstract】 In order to screen the optimal genotypes for embryonic tip regeneration system in soybean(Glycine max L.Merr.),the embryonic tips of five soybean cultivars including Jidadou 1 and Jidadou 2,were used as explants to study the effects of 6-benzyl aminopurine(6-BA)concentration on induction and regeneration rate of adventitious bud.The screened soybean cultivar Jidadou 2,fitting for soybean embryonic tip regeneration system,were used as explants to study the influence of Indole-3-butytric acid(IBA)on browning rate of embryonic tips when adding proline and silver nitrate.The browning rate was effectivly inhibited when the concentration of 3.0 mg·L-1 proline and 3.0 mg·L-1silver nitrate were as the additives in co-cultivation medium.Sucrose with a high concentration of IBA could increase the rooting ratio.更多还原