【摘要】 为明确转基因大豆的种植及生态安全性,将导入抗逆基因TaDREB3的转基因大豆T4代播种于大田,周围种植非转基因对照,收获转基因大豆周围不同距离的非转基因大豆种子。经过连续2 a的田间及盆栽试验,通过除草剂草丁膦的筛选和分子检测等手段,研究了转TaDREB3基因大豆的基因漂流距离及漂流频率。结果表明:在三叶期整株喷洒75 mg·L-1的草丁膦是较适宜的抗性植株筛选浓度;在自然条件下漂流距离为1 m的非转基因大豆中检测到1条阳性带,漂流距离为3、5和8 m的非转基因大豆均未检测到阳性带,确定转TaDREB3基因大豆基因漂流距离为1 m,基因漂流频率为0.0348%。更多还原

【Abstract】 In order to investigate the ecological safety issues associated with transgenic soybeans planting,seeds of T4 generation of transgenic lines overexpressing TaDREB3,a stress-tolerant gene,were sowed in the field,surrounded with the nontransgenic control plant.The distance and frequency of gene flow in transgenic soybean overexpressing TaDREB3 were determined.Two successive years’ field and potted tests were carried out through glufosinate selection as well as molecular detection.The results showed that the optimal concentration of glufosinate selection in the trifoliate stage was 75 mg·L-1.One positive plant was detected in the nontransgenic soybean at 1 m distance from the transgenic plant center,while no positive was detected in 3,5 and 8 m plants.Therefore,the possibility of TaDREB3 gene flow under nature condition was 0.03478% with 1 m distance.更多还原