【摘要】 以适宜转化大豆受体YC-2为材料,研究了抗氧化剂α-硫辛酸对农杆菌介导GUS瞬时表达和芽诱导率的影响。结果表明:在共培养基中添加不同浓度的抗氧化剂可显著降低外植体的褐化率,随α-硫辛酸浓度的增加外植体褐化率明显减少,但白化率增加,且这些白化的外植体在后期不易产生诱导芽;共培养中添加不同浓度的α-硫辛酸可增强GUS瞬时表达率和芽诱导的再生率,且以25 mg·L-1效果最佳。更多还原

【Abstract】 Antioxidant-α-lipoic acid(LA)influencing on the Agrobacterium-tumefaciens mediated GUS transient expression rate and shoot induction percentage in soybean cultivar’YC-2’was studied in this paper.The results showed the explant browning percentage was markedly decreased after added different concentrations of α-lipoic acid in the co-cultivation medium.In the meantime,the explant whitening percentage was increased with the increased concentration of α-lipoic acid in co-cultivation medium.Meanwhile,the whitening explant couldn’t induce shoot regeneration after culured on the shoot induction medium for a month.It also showed the transient GUS expression rate and shoot induction frequency were also increased after added different LA concentrations in the co-cultivation medium.The optimized LA concentration was 25 mg·L-1in the co-cultivation medium.更多还原