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广西新收集野生大豆资源的遗传多样性分析

Genetic Diversity Analysis of New Wild Soybean Collection in Guangxi

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【作者】 曾维英梁江陈渊韦清源汤复跃钟开珍陈文杰

【Author】 ZENG Wei-ying1,2,LIANG Jiang1,CHEN Yuan1,WEI Qing-yuan1,TANG Fu-yue1,ZHONG Kai-zhen1,CHEN Wen-jie1(1.Guangxi Maize Research Institute,Nanning 530227;2.Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China)

【机构】 广西玉米研究所广西农业科学院作物遗传改良生物技术重点实验室

【摘要】 利用23对SSR引物对广西新收集的200份野生大豆资源进行遗传多样性分析。结果表明:23个SSR位点共扩增出137个多态性带,每个位点等位变异数目范围为3~11个,平均为5.96个;Simpson指数分布范围为0.503~0.834,平均为0.689;Shannon-weaver指数分布范围为0.407~1.444,平均为0.683;遗传相似系数分布范围为0.266~0.969,平均为0.720,广西新收集野生大豆材料表现出丰富的遗传多样性。聚类分析结果显示,200份材料分为三大类,各地区材料之间存在着明显的遗传差异,但有些同一地区内的材料也表现出了明显的遗传分化。

【Abstract】 Two hundred accessions of wild soybean,collected from Guangxi province were used as materials in this study.Twenty-three pairs SSR primers were used to analyze the genetic diversity of these materials.The result showed that one hundred and thirty-seven bands were amplified in twenty-three pairs SSR primers,with each locus has 5.96 bands.Genetic diversity index showed that distribution of Simpson index was 0.503-0.834,distribution of Shannon-weaver index was 0.407-1.444,and the dicesimilarity was 0.266-0.969,which suggestted that wild soybean collected from Guangxi had abundant genetic diversity.Clustering analysis showed that,two hundred accessions of wild soybean were divided into 3 categories.The genetic distance was very close among the materials in one population and also the materials of different populations because of their similar habitat.However,there were genetics differentiations in a same population.

【关键词】 广西野生大豆遗传多样性SSR
【Key words】 GuangxiWild soybeanGenetic diversitySSR
【基金】 广西区青年科学基金资助项目(桂科青099171);广西农业科学院基本业务费[200814(基)]现代农业产业技术体系建设专项资金资助项目
  • 【分类号】S565.1
  • 【被引频次】3
  • 【下载频次】113
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