【摘要】 以吉林35、吉林47等5个大豆品种为材料,胚尖为外植体,研究不同浓度6-BA对丛生芽诱芽率和平均芽数的影响,同时测定吉林35在胚尖诱导丛生芽的过程中对潮霉素的耐受性。结果表明:3.0 mg.L-16-BA浓度下吉林35的诱芽率最高,综合考虑丛生芽诱芽率和平均芽数2个性状,吉林35更加适合于大豆胚尖再生系统;在遗传转化中,以3.0 mg.L-1潮霉素作为吉林35抗性筛选的适宜浓度。更多还原

【Abstract】 In order to establish an efficient embryonic tip regeneration system for soybean(Glycine max L.Merr.),five soybean cultivars,including Jilin 35,were used as explants to study the effects of 6-BA and genotypes on the numbers and induction rate of adventitious shoots.The endurance of the induced shoots of Jilin 35 to Hygromycin B was also studied.The results showed that Jilin 35 was fit for soybean embryonic tip regeneration system according to the numbers and inductivity of adventitious shoots.The concentration of 3.0 mg·L-1 Hygromycin B was advised for resistance screening in embryonic tip regeneration system.更多还原