【摘要】 曲古抑菌素A(Trichostatin A,TSA)是一种组蛋白去乙酰化酶抑制剂。有研究表明TSA处理可以提高核移植胚胎的发育率。为探讨TSA对猪核移植胚胎发育的作用,试验重点研究了TSA浓度及处理时间对核移植胚胎体外发育的影响,同时也探索了TSA对不同供核细胞构建的重构胚体外发育的影响。结果表明,40nmo·lL-1的TSA处理核移植胚胎可以显著提高其体外囊胚发育率;40nmo·lL-1TSA处理重构胚12和24h,两组重构胚的卵裂率与囊胚发育率差异不显著;40nmo·lL-1TSA处理12h对不同供核细胞构建的重构胚均具有提高囊胚发育率的效果,不同供核细胞所构建的重构胚发育率无显著差异。由此可知,40nmo·lL-1TSA处理12或24h可以提高猪体细胞核移植胚胎体外发育能力。更多还原

【Abstract】 Histone deacetylase inhibitors (HDACi),such as trichostatin A (TSA),can significantly enhance the developmental competence of somatic cell nuclear transfer (SCNT) embryos.The present study was designed to examine the effect of TSA,especially the concentration of TSA and the treat period,on the development of pig SCNT embryos.The reaults showed that the treatment of cloned pig embryos with 40 nmol·L-1 TSA could significantly improve the in vitro blastocyst rate compared with the control group,whereas no significant deference of the blastocyst rate was observed between treatment period of 12 and 24 h.40 nmol·L-1 TSA treated 12 h could enhance the blstocyst rate of porcine SCNT embryos regardless of the difference of donor cells compared with control group,but there was no significant difference of the blastocyst rate between SCNT embryos reconstructed with diffirent donor cells.These results indicated that 40 nmol·L-1 TSA treated 12 or 24 h could enhance the porcine SCNT embryos in vitro development competence.更多还原