【摘要】 采用HPLC、聚酰胺树脂、HPTLC等技术手段,进行EGCG3"Me的提取与分离纯化研究。通过正交实验表明,EGCG3"Me最优提取工艺条件为:乙醇体积分数70%、回流时间30 min、回流温度80℃、料液比1∶10,质量分数为0.89%。采用40%酒精、60%酒精进行冼脱时,有大量EGCG3"Me富集。以甲醇∶氯仿=7∶2为洗脱剂,采用100～120目聚酰胺树脂进一步分离,可以得到纯度约为40%的EGCG3"Me。更多还原

【Abstract】 The extraction and separation process of EGCG3"Me was studied by HPLC,polyamide resin and HPTLC methods.According to the orthogonal experimental design,the optimum extracting conditions of EGCG3"Me were as follows: alcohol concentration 70%,reflux time 30 min,reflux temperature 80℃ and solid-liquid ratio 1:10.And the yield of EGCG3"Me was 0.89%.Large amount of EGCG3"Me was found in the elution of alcohol concentration 40% and 60%.With elution of methanol: chloroform(7:2),the high purity EGCG3"Me was obtained by further separation and purification of 100～120 mesh polyamide resin.更多还原