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溶菌酶产生菌的筛选及产酶条件研究

Isolation of high-yielding lysozyme strain and optimization of its fermentation conditions

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【作者】 李潜于宏伟郭润芳贾英民

【Author】 LI Qian1,YU Hong-wei1,GUO Run-fang1,JIA Ying-min1,2 (1.College of Food Science and Technology,Agricultural University of Hebei,Baoding 071001,China;2.College of Biology Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050000,China)

【机构】 河北农业大学食品科技学院河北科技大学生物科学与工程学院

【摘要】 为了从微生物中获取溶菌酶,本研究采用双层平板法从土壤中筛选获得一株产溶菌酶活性较高的菌株S2-6b。利用摇瓶发酵优化产酶条件,液体发酵培养基成分为可溶性淀粉1.5%,牛肉膏2.5%,玉米浆0.5%,NaCl 0.5%,起始pH值为7.0,培养温度为28℃,接种量为3.0%,摇瓶的装液量为40 mL/300 mL,转速为200r/min。培养40 h酶活力最高,最高酶活力可达6 167 U/mL。

【Abstract】 In order to obtain lysozyme from microorganism,a high-yielding strain for producing lysozyme was screened by double layer plates.The condition for enzyme formation of the strain was studied.The suitable carbon source of medium was soluble starch and the suitable nitrogen source was meat extract and corn steep liquor,whose suitable concentration was 1.5%,2.5% and 0.5% respectively.The optimum temperature and initial pH for enzyme formation were 28 ℃ and pH 7.0 respectively.The inoculum size,liquid volume and shake speed were 3.0%(V/V),40 mL/300 mL 200 r/min respectively.The fermentation cycle was 40 h and the highest lysozyme activity was 6 167 U/mL.

【关键词】 溶菌酶筛选产酶条件
【Key words】 lysozymescreenfermentation conditions
【基金】 河北省十一五科技计划项目:农产品精深加工用主要微生物菌种培育及菌种资源库建设(06220106D-1)
  • 【文献出处】 河北农业大学学报 ,Journal of Agricultural University of Hebei , 编辑部邮箱 ,2011年01期
  • 【分类号】TQ925
  • 【被引频次】5
  • 【下载频次】500
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