【摘要】 目的研究白细胞介素-2(IL-2),IL-2及白细胞介素-12(IL-12),IL-2及白细胞介素-18(IL-18),IL-2、IL-12及IL-18活化的自然杀伤细胞(NK细胞)的形态及对黑色素瘤细胞的杀伤率。方法提取NK细胞,IL-2 6 000 I U/mL,IL-2 6 000 I U/mL+IL-12 10 ng/mL,IL-2 6 000 I U/mL+IL-18 100 ng/mL,IL-2 6 000 I U/mL+IL-12 10 ng/mL+IL-18 100 ng/mL活化,3 d后流式细胞仪检测其前向角散射(FSC)值,酶联免疫吸附法(ELISA)检测干扰素γ(IFNγ)含量,以B16黑色素瘤细胞为靶细胞检测NK细胞对肿瘤细胞杀伤率。结果 IL-2+IL-12、IL-2+IL-18、IL-2+IL-12+IL-18活化的NK细胞FSC值明显比仅用IL-2活化的NK细胞大(P<0.05),并且分泌的IFN量高(P<0.05),但对B16黑色素瘤细胞杀伤率低。结论同时应用IL-12及IL-18与IL-2活化的NK细胞能够分泌较高的IFNγ,但是对肿瘤细胞的杀伤作用较低。更多还原

【Abstract】 Objective To investigate shape and function of different cytokines activated NK cells.Methods Natural Killer(NK)cells were cultured and activated with IL-12 10 ng/mL,IL-18 100 ng/mL,and both with IL-2 6 000U/L,3 days later took out NK cells,markers and Forward Scatter(FSC) FACS were detected by FACS,IFN gamma production was detected by ELISA,and B16 melanoma cells as targets for NK cells for cytotoxic assay.Results IFN gamma production from NK cells with IL-2+IL-12,IL-2+IL-18,and IL-2+IL-12+IL-18 were higher and more than with IL-2 respectively,but NK cells with IL-2+IL-12,IL-2+IL-18,and IL-2+IL-12+IL-18 kill less B16 cells than with IL-2.Conclusion NK cells with IL-2+IL-12,IL-2+IL-18,and IL-2+IL-12+IL-18 will produce more IFN gamma,but kill less tumor cells.更多还原