【摘要】 目的探讨缺氧性脑损伤大鼠痫性发作敏感性及其囊泡膜谷氨酸转运载体-1(VGluT1)、囊泡膜谷氨酸转运载体-2(VGluT 2)表达变化。方法成年雄性SD大鼠随机分为对照组(n=35)和缺氧组(n=35)。两组各取10只大鼠腹腔注射戊四唑(pentylenetetrazol,PTZ)10 mg/kg,5 min检测致痫性发作阈值;两组再各取15只大鼠腹腔注射PTZ 35 mg/kg,2 d,连续20 d点燃大鼠,检测点燃大鼠数目和痫性发作程度,两组中经PTZ点燃大鼠分别纳入单纯点燃组和缺氧后点燃组;采用免疫组织化学和免疫印迹观察对照组(n=10)和缺氧组(n=10)与单纯点燃组和缺氧后点燃组大鼠颞叶皮层、海马和中脑VGluT1、VGluT2表达的变化。结果缺氧损伤后大鼠癫痫敏感性明显增强。缺氧组颞叶皮层和海马区VGluT1表达高于对照组;而缺氧后点燃组颞叶皮层和海马区VGluT1表达较缺氧组和单纯点燃组明显增加(P<0.05)。各组海马与中脑VGluT2表达差异无统计学意义(P>0.05)。结论成年大鼠缺氧性脑损害后癫痫敏感性增强,其可能与VGluT1表达变化有关。更多还原

【Abstract】 Objective To explore seizure suscepitibility in rats following hypoxic brain injury and vesicular glutamate transporter subtype 1(VGluT1) and vesicular glutamate transporter subtype 2(VGluT 2)expression.Methods SD male rats were assigned to the control rats(n=35)and the rats exposured to global hypoxia induced by 8% nitrogen oxygen atmosphere(n=35).To test the seizure threshold,rats from both groups(n=10)were treated with pentylenetetrazol(PTZ 10mg/kg.5min)intraperitoneally.To test the number of kindled rats and seizure scales,other rats from both groups(n=15)were injected intraperitoneally subconvulsive dosage of PTZ(35 mg/kg.2d)for 20 d,successively,and then rats kindled by PTZ in both groups were divided into simple kindling group and post-hypoxic kindling group,respectively.Furthermore,VGluT1 and VGluT2 expressions in the temporal cortex,midbrain,and hippocampal were studied in the rest of both group(n=10)and the rats kindled by PTZ in both groups by using immunohistochemistry and immunoblotting,respectively.Results The rats with exposure to hypoxia exhibited increased seizure susceptibility to PTZ.VGluT1 expression within temporal cortex and hippocampus regions in hypoxic group were obviously higher than that in control group.Compared with hypoxia and simple kindling groups,VGluT1 expression was markedly enhanced in posthypoxic kindling group(P<0.05).There was no significant difference in VGluT2 exression in each groups(P>0.05).Conclusion The seizure susceptibility in adult rats following hypoxic cerebral is increased,while the increased expression of VGluT1 may lead to hyperexcitability in brain,which may play an important role in the alterations of seizure susceptibility following hypoxic cerebral injury.更多还原