【摘要】 选择等离子体对乙丙交酯共聚物(PLGA)表面进行活化处理来接枝胶原。通过测定等离子体处理后PLGA材料表面水接触角确定最佳处理功率为60W,时间5min。XPS分析确定胶原表面的羧基与PLGA表面等离子体激活的羟基发生了反应生成酯基,而使胶原接枝到PLGA表面。选用小鼠胚胎内皮成纤维细胞(3T3)在PLGA材料表面培养。实验定性观察确定胶原接枝有利于细胞的黏附和增殖。采用四甲基偶氮唑蓝比色法(MTT)对不同时期两种细胞生长情况进行定量分析,结果表明,接枝胶原的存在改善了细胞黏附作用,加快了细胞的增殖速度。更多还原

【Abstract】 For collagen is a major component of the extracellular matrix,when the surface of poly(lactid-glycolide acid)(PLGA) is grafted with collagen,cytocompatibility of PLGA will be improved.Plasma treatment is selected to immobilize collagen on the surface of PLGA.Optimum power and treating time of plasma are determined by contact angle test.The results indicate they are 60W and 5min,respectively.XPS analysis reveals that carboxy groups on collagen are reacted with actived hydroxyl groups on the surface of plasma treated PLGA to form ester groups.Then collagen is immobilized on PLGA.Cytocompatibility evaluation of collagen immobilized PLGA(COL-PLGA) was carried out by seeding 3T3 cells on the surface of COL-PLGA.Quanlitative and quantitative analysis proved that cell adhesion and proliferation are improved after collagen grafting.Quantitative result is obtained by MTT assay in cell living period.更多还原