【摘要】 目的:研究天花粉蛋白(TCS)对子宫颈癌Hela细胞Survivin基因表达的影响,以探讨TCS诱导Hela细胞凋亡的机制。方法:MTT法检测TCS对Hela细胞增殖的影响,倒置显微镜观察细胞形态;RT-PCR和Western blot分析TCS对Hela细胞Survivin基因表达的影响;流式细胞仪分析TCS对Hela细胞周期的影响。结果:①TCS显著性抑制Hela细胞增殖;②TCS处理后Hela细胞Survivin mRNA水平无明显改变,但蛋白表达水平显著降低;③TCS降低Hela细胞周期中G2/M期细胞比率,但增加S期细胞的数量。结论:通过下调凋亡抑制因子Survivin的表达而诱发Hela细胞凋亡,可能是TCS抑制Hela细胞增殖的分子机制。更多还原

【Abstract】 Objective: To observe the effects of Trichosanthin(TCS) on expression of Survivin gene in Hela cell line and to explore the underlying molecular mechanism on the growth inhibition and the apoptosis of Hela cell line induced by TCS.Methods: MTT assay was used to determine the effect of TCS on cell proliferation;the cell morphology was observed by inverted microscope.RT-PCR and Western-blot assay were used to detect expression level of Survivin gene in Hela cells after TCS treatment.The FCM analysis was carried out to examine the effect of TCS on the cell cycle of Hela cells.Results: ①The proliferation of Hela cells was significantly inhibited by TCS;②TCS treatment decreased expression of Survivin in the protein level,but no change was observed in the mRNA level;③TCS treatment resulted in deceased cell number in G2/M phase but increased cell number in S phase.Conclusion: TCS inhibits the growth of Hela cells through decreasing the expression of Survivin,which results in activation of apoptosis pathways.更多还原