【摘要】 目的:研究大肠癌虚证(气血亏虚)和实证(湿热蕴结)蛋白质差异性表达,从蛋白质水平上探讨大肠癌虚实证的本质。方法:用双向凝胶电泳分离虚实证大肠癌蛋白质,银染,PDQuest 2-DE软件分析,对部分差异蛋白质点进行基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)测定其胶内酶解后的肽质指纹图谱,查询SWISS-PROT、NCBI数据库。结果:获得了分辨率和重复性均较好的双向电泳图谱,共筛选出23个差异蛋白点,其中有21个差异蛋白质得到鉴定,这些蛋白包括热休克蛋白、细胞骨架蛋白、抗氧化蛋白、信号转导相关蛋白、能量产生相关蛋白、血液蛋白等。结论:初步建立了大肠癌虚实不同证型-蛋白质表达谱,为研究大肠癌虚实不同证型的本质提供了物质基础。更多还原

【Abstract】 Objective:To investigate the different expression of proteins between deficiency syndromed(qi-xue deficiency type) and excess syndrome(shi-re type) of colorectal cancer,So as to reveal the essence of the two TCM syndromes of colorectal cancer from the protein expression level.Methods:A series of methods,including two-dimensional electrophoresis(2-DE),silver nitrate stain,PDQuest 2-DE analysis software,peptide massfingerprint based on matrix-assisted laser desorption/ionization time of flying mass spectrometry(MALDI-TOF-MS) and SWISS-PROT,NCBI database searching,were used to separate and identify the proteome of between deficiency and excess syndrome of colorectal cancer.Results:The good 2-DE image including resolution and reproducibility was obtained.23 differential expression protein spots were screened,then 21 proteins were identified,which related to heat shock protein,cytoskeletal protein,antioxidation protein,signal transduction related protein,energy production related protein and blood protein,etc.Conclusions:Excess and deficiency syndrome of colorectal cancer-protein expression profiling was established initially,which was helpful to provide substance evidence in investigation of the essence of deficiency syndrome and excess syndrome of colorectal cancer.更多还原