【摘要】 目的:观察博莱霉素致肺纤维化大鼠肺组织中肿瘤坏死因子-α(TNF-α)及mRNA的表达,探讨中药复方参龙煎剂防治大鼠肺纤维化的作用机制。方法:160只健康雄性Wistar大鼠随机分为正常对照组、模型组、泼尼松组、参龙煎剂组,采用气管内注入博莱霉素建立Wistar大鼠肺纤维化模型。正常对照组和模型组大鼠第2d起每日予生理盐水灌胃,参龙煎剂组大鼠按配好的参龙煎剂以等容药品灌胃。免疫组化检测大鼠肺组织TNF-α蛋白的表达,逆转录聚合酶链式反应检测大鼠肺组织TNF-αmRNA的表达水平。结果:参龙煎剂可能参与调控肺组织TNF-α/TNF-αmRNA的表达,除正常对照组外,其他各组大鼠第7d时TNF-α和TNF-αmRNA表达均明显增强,7d、14d各治疗组与正常对照组比较均有显著性差异(P<0.05或P<0.01),两用药组表达明显低于模型组,比较差异均有显著性(P<0.01);两治疗组间比较均无显著性差异;28d时,模型组仍有明显的强势表达,与其他各组比较仍存在显著差异(P<0.05或P<0.01)。结论:实验研究表明参龙煎剂可能是通过调控TNF-α/TNF-αmRNA的表达,起到延缓肺纤维化的作用。更多还原

【Abstract】 Objective: To observe the expression of tumor necrosi factor(TNF-α)/ mRNA in the rat model of bleomycin induced pulmonary fibrosis,and to discuss the roles which playing in injury and reparative process,illuminate the mechanism of action of Shenlong Decoction.Methods: 160 Wistar rats were divided into normal group,model group,prednisone group,Shenlong Decoction group.Wistar rats were intratracheally administered bleomycin to establish pulmonary fibrosis model,from 2nd the normal group and model group rats were lavaged into stomach with ns,Shenlong Decoction group groups were lavaged with prepared Shenlong decoction by the same volume.Expressions of tumor necrosi factor(TNF-α) in the lung homogenate were measured by immunohistochemistry,expressions of tumor necrosi factor(TNF-α)/mRNA in the lung homogenate were measured by reverse transcription-polymerase chain reaction(PCR).Results: Shenlong decoction may join in the expressive regulation of TNF-α/TNF-αmRNA,except for the normal group,the expressions of the other groups,of TNF-α/TNF-α were boost up distinctly on the 7th day,compared with the normal group on the 7th and 14th,all the treatment groups,had significant difference(P<0.05 or P<0.01),compared with the model group,all the treatment groups,expression decreased obviously,the compared difference was significant(P<0.01);compared with the treatment groups,the difference was not significant;the model group still had strong tendency of expressive on the 28th day,compared with the other groups,the difference was still significant(P<0.01or P<0.05)Conclusion: Experimental study indicated that Shenlong decoction may play the role of staving pulmonary fibrosis by regulating the expression of TNF-α/TNF-αmRNA.更多还原