【摘要】 目的:探讨轮叶党参总皂苷(CLTS)对人肝癌HepG-2细胞的抑制作用,阐明其凋亡调控作用机制。方法:HepG-2细胞分为100、200、300、400、500、600mg·L-1 CLTS处理组和对照组(未加CLTS),处理HepG-2细胞72h后,采用MTT法检测细胞生长抑制率,计算半数抑制浓度(IC50);HePG-2细胞分为100、150、200mg·L-1 CLTS处理组和对照组(未加CLTS),处理HepG-2细胞72h后,用流式细胞仪检测细胞凋亡率,用酶标仪检测caspase-3,8,9活性。结果:CLTS处理组随着浓度的增加,HepG-2细胞的生长抑制率增加,并呈时间-剂量依赖性(P<0.01),IC50值为239.623mg·L-1;CLTS不同浓度处理组细胞凋亡率明显高于对照组(P<0.01);随着CLTS浓度的增加,caspase-8,9蛋白活性明显增强,呈剂量依赖性(P<0.01)。结论:轮叶党参总皂苷对人肝癌HepG-2细胞的生长具有抑制作用。通过上调caspase 8,9蛋白表达,激活caspase-3,从而诱导细胞凋亡可能是其作用机制之一。更多还原

【Abstract】 Objective To investigate the inhibitory effect of Codonopsis lanceolata total saponin(CLTS) on human hepatoma HepG-2 cells and clarify its regulatory effect on apoptosis.Methods The HepG-2 cells were divided into 6 treatment groups(100,200,300,400,500,and 600 mg·L-1 CLTS) and control group.The inhibitory rate of HepG-2 cell growth was detected by MTT assay and the IC50 value was calculated after treated for 72 h.The HepG-2 cells were divided into 3 treatment groups(100,150 and 300 mg·L-1 CLTS) and control group.The apoptotic rate was determined by flow cytometry,the activities of cspase 8,9 and 3 in carcinoma cells were determined by microplate reader.Results With the increasing of the concentration of CLTS,the inhibitory rate of HepG-2 cell growth was increased,the IC50 value was 239.623 mg·L-1,the different concentrations of CLTS inhibited the HepG-2 cells in dose-and time-dependent manner(P<0.01).The apoptotic rates in treatment groups were significantly higher than that in control group(P<0.01).The activities of Caspase-8,9 and 3 were remarkably increased after treated with CLTS compared with control group in a dose-dependent manner(P<0.01).Conclusion CLTS could significantly inhibit the growth of HepG-2 cells.The induction of apoptosis through up-regulating caspase-8 and 9,then activating caspase-3 is probably one of its molecular mechanisms.更多还原