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黄连木组培快繁育苗研究

On rapid propagation for tissue culture seedlings of Pistacia chinensis

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【作者】 何敬房苏淑钗冷平生姬谦龙于涌鲲刘文科

【Author】 HE Jing-fang1a,SU Shu-chai3,LENG Ping-sheng1a,JI Qian-long1b,YU Yong-kun1,LIU Wen-ke3(1a.College of Landscape and Forestry,1b.Center of Tissue Culture,Beijing University of Agriculture,Beijing102206,China;2.College of Forestry,Beijing Forestry University,Beijing 100083 China;3.Institute of Agricultural Environment and Sustainable Development,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

【机构】 北京农学院园林学院北京林业大学林学院北京农学院组培中心中国农业科学院农业环境与可持续发展研究所

【摘要】 以黄连木的枝条作为外植体进行组织培养。结果表明:1/2 DKW+6-BA 1.0 mg/L+NAA 0.1 mg/L+IBA 0.2 mg/L作为茎段腋芽诱导的培养基,芽体长达4 cm;1/2 DKW+6-BA 4.0 mg/L+IBA 0.04 mg/L+NAA 0.02 mg/L为增殖培养基,增殖系数可达4.21;1/2 DKW+IBA 6.0 mg/L为生根培养基,生根率达到91.15%;移栽在草炭∶蛭石∶粗砂=3∶1∶2混合基质中,成活率高达92.11%以上。

【Abstract】 The stem-segments were used as explants in the tissue cultrue of Pistacia chinensis Bunge.Results indicated that:Medium of 1/2DKW+6-BA 1.0 mg/L+NAA 0.1 mg/L+IBA 0.2 mg/L was the best medium for the induction of axillary bud,and the bud’s length was up to 4cm;Multiplication coefficient was 4.21 on the medium of 1/2 DKW+6-BA 4.0 mg/L+ IBA 0.04 mg/L+NAA0.02 mg/L IBA 0.1 mg/L;Rooting rate was up to 91.15% on the medium of 1/2DKW+ IBA6.0 mg/L;The suitable transplantation martix was peat∶verimiculite∶coarse sand(3∶1∶2),and survial rate of plant with root was above 92.11%.

【关键词】 黄连木组织培养快速繁殖生根
【Key words】 Pistacia chinensistissue culturerapid propagationrooting
【基金】 北京市自然科学基金项目(6072005);北京市创新人才建设计划项目(PXM2009-014207-076874);北京农学院组培中心资助项目
  • 【文献出处】 北京农学院学报 ,Journal of Beijing University of Agriculture , 编辑部邮箱 ,2011年03期
  • 【分类号】S792.99
  • 【被引频次】6
  • 【下载频次】262
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