【摘要】 目的了解广州地区无偿献血人群丙型肝炎病毒部分基因的核苷酸序列和基因型分布。方法收集广州地区无偿献血人群中抗-HCV阳性标本201份,采用荧光定量PCR(Q-PCR)的方法对其进行核酸检测,阳性标本同时作HCVE1和NS5B基因扩增;核苷酸序列测定后运用DNASTAR,B ioEd it,M ega4.0等软件作序列分析和基因分型。结果 201份抗-HCV阳性标本的HCV RNA阳性率为54.23%(109/201),其中男性的HCV RNA阳性率为63.19%(91/144)、女性为31.58%(18/57)(P<0.05)。109份HCV RNA阳性的标本全部扩增出E1和NS5B基因,基因分型显示其HCV基因型比例依次为1b(46.79%)、6 a(33.03%)、3 a(10.09%)、2 a(5.50%)、3b(3.67%)、1 a(0.92%)。结论广州地区抗-HCV阳性的无偿献血人群HCV RNA阳性率男性高于女性,HCV毒株以1b和6 a亚型最多见。更多还原

【Abstract】 Objective To determine the sequence of HCV partial genes and the distribution of HCV genotypes among volunteer blood donors in Guangzhou.Methods A total of 201 anti-HCV(+) plasma samples were obtained from volunteer blood donors in Guangzhou.HCV RNA was detected by fluorescent quantitation polymerase chain reaction(Q-PCR).The positive samples were tested by RT-PCR for E1 gene and NS5B gene,followed by direct DNA sequencing and phylogenetic analysis by DNASTAR,BioEdit and Mega4.0 softwares.Results HCV RNA was positive in 109 samples out of 201 Anti-HCV(+) plasma samples.The overall positive rate of HCV RNA was 54.23%(109/201) among anti-HCV(+) samples,with 63.19%(91/144)in males and 31.58%(18/57) in females(P< 0.05).Genotypes were determined for the 109 HCV RNA positive samples(both based on E1 gene and NS5B gene).Among these samples,subtypes 1b,6a,3a,2a,3b,and 1a were detected at frequencies of 46.79%,33.03%,10.09%,5.50%,3.67%,and 0.92%,respectively.Conclusion The positive rate of HCV RNA in males was higher than in females.And 1b and 6a were the predominant subtypes among volunteer blood donors in Guangzhou.更多还原