【摘要】 通过调节进水ρ(P)ρ/(C)的不同水平(2.9/100、1.4/100、0.57/100、0.29/100、1.4/100和2.9/100),考察了A/O-SBR系统强化生物除磷效果的动态变化;同时利用PCR-DGGE分子生物学技术,研究了聚磷菌和聚糖菌种群的竞争与演变.结果表明,当ρ(P)ρ/(C)逐渐降低时(2.9/100→1.4/100→0.57/100→0.29/100),吸收单位碳源的厌氧释磷量逐渐降低,而胞内糖原量逐渐升高.相应的DGGE图谱显示,微生物类群在ρ(P)/ρ(C)降低过程由11 OTUs升高到14 OTUs,最后降至7 OTUs;结合生化指标判断,系统优势菌种呈现的是从聚磷菌占优势、聚糖菌聚磷菌共存到聚糖菌占优势的动态变化.随后,提高进水ρ(P)ρ/(C)值从0.29/100到1.4/100再到2.9/100,污泥吸收单位碳源的厌氧释磷量逐渐升高,而胞内糖原量逐渐降低.这说明当聚糖菌占优势以后,通过调节ρ(P)ρ/(C)可重新培养获得聚磷菌优势系统,但DGGE图谱也显示,此时的聚磷菌优势种群较聚糖菌系统的优势种群已有较大变化,且与先前聚磷菌系统的优势种群也不尽相同.更多还原

【Abstract】 The effect of the different P/C ratios,i.e.,2.9/100,1.4/100,0.57/100,0.29/100,1.4/100 and 2.9/100,on the dynamic changes of the phosphorus removal as well as the organism community was examined in an anaerobic/aerobic sequencing batch reactor(A/O-SBR) system,affiliated with Polymerase Chain Reaction-denaturing Gradient Gel Electrophoresis(PCR-DGGE).Results showed that when the P/C ratio dropped(2.9/100→1.4/100→0.57/100→0.29/100) gradually,the P-release to substrate-uptake ratios decreased companied by an increase in the intracellular glycogen content.Also,the DGGE profiles showed that with the decrease of the influent P/C ratios the dominant microbial species increased from 11 operation taxonomy units(OTUs) to 14 OTUs,and then dropped to 7 OTUs.Judged from the biochemical characteristics of the SBR,it can be confirmed that the predominant microbial populations in the SBR had changed from PAOs to PAOs and GAOs co-exist,and finally to GAOs.For the last recovery period,the P-release to substrate-uptake ratios increased gradually with the decrease of the intracellular glycogen content,indicating that PAOs can be re-cultivated through regulating the influent P/C ratio,once GAOs had dominated the EBPR system.While,the DGGE profiles showed that the dominant species of PAOs re-cultivated from the GAOs-dominated system was different greatly from both the GAOs-dominated system and the initial PAOs-dominated system.更多还原