【摘要】 细胞色素c在细胞凋亡中发挥着重要的作用,其作用机理在高等真核生物及低等真核生物酵母中已经比较清楚,但在盘基网柄菌(Dictyostelium discoideum)中的作用却没有相关报道。所以我们用western blot和实时荧光定量PCR的方法分别测定了盘基网柄菌前柄细胞和前孢子细胞中细胞色素c的含量及表达量的变化,来探讨细胞色素c在盘基网柄菌前柄细胞凋亡中的作用。结果显示,①凋亡的前柄细胞发育16～20 h的过程中,线粒体内细胞色素c含量呈递减趋势,与之相反的是胞质中细胞色素c的含量却呈现出递增趋势。前柄细胞细胞色素c总含量高于前孢子细胞。②前柄细胞中细胞色素c基因的表达水平明显高于前孢子细胞,并且呈现出递增的趋势,而在前孢子细胞中细胞色素c基因的表达量比较稳定。两个研究结果相互印证,说明细胞色素c在一定程度上参与了盘基网柄菌前柄细胞的凋亡过程。更多还原

【Abstract】 Cytochrome c plays an important role in apoptosis of eukaryotes and yeast,but there are few reports about its role in Dictyostelium discoideum.Western blot and S Fluorescence Quantitative PCR were used to detect the quantity and expression changes of cytochrome c in prestalk cells and prespore cells,in order to reveal the role of cytochrome c in apoptosis of dityostelium prestalk cells.In process of 16-20 h development of apoptotic prestalk cells,the amount of mitochondrial cytochrome c showed a decreasing tendency while the amount of cytoplasmic cytochrome c showed an increasing tendency inversely.Total amount of cytochrome c in prestalk cells was higher than that in prespore cells.Cytochrome c gene expression in prestalk cells was higher than that in prespore cells,and showed an increasing tendency,but cytochrome c gene expression in prespore cell was relatively stable.These results indicate that cytochrome c,to some extent,participates in the apoptosis of Dictyostelium prestalk cells.更多还原