【摘要】 为了确定饲料中胆固醇含量对雄性红螯光壳螯虾(Cherax quadricarinatus)生长和生殖的影响,本研究通过对初始体长为10 cm左右的雄虾投喂胆固醇添加量分别为对照组(0%)、实验1组(0.25%)、实验2组(0.50%)、实验3组(0.75%)、实验4组(1.00%)的人工配合饲料10周后,测定各组实验虾的肝胰腺消化酶、精巢碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)和ATP酶活力。结果显示,生长指标和胰蛋白酶活力以实验2组最高;脂肪酶活力实验2、3、4组之间无显著差异,但显著高于实验1组和对照组;精巢AKP活力和LDH活力均以实验3组最高;精巢ATP酶活力实验2组和实验3组之间无显著差异,均显著高于实验1、4组和对照组。结果表明,对处于性腺快速发育阶段的雄性红螯光壳螯虾饲料中胆固醇的最适添加量在0.50%～0.75%之间。更多还原

【Abstract】 The male Redclaw Crayfish(Cherax quadricarinatus) with initial body length of 10.5 cm were fed with artificial diets containing different levels of cholesterol(0,0.25%,0.50%,0.75%,1.00%) for 10 weeks.Hepatopancreas digestive enzymes and testis AKP,LDH,ATPase were measured.The results showed that group 2 had the best growth index and the highest trypsin activity.There was no significant difference in lipase activity among groups 2,3,and 4,but lipase activities in these three groups were higher than those in group 1 and control.Testis AKP activity and LDH activity were the highest in group 3.There was no significant difference in testis ATPase activity between group 2 and 3,but ATP activities in these two groups were higher than those in groups 1,4 and control.The results suggest that the optimum dietary cholesterol in the feed was between 0.50% to 0.75% during rapid gonadal development phase in C.quadricarinatus.更多还原