【摘要】 以勋章菊无菌苗叶片为外植体,以MS为基本培养基,添加不同浓度TDZ、2,4-D、IAA、IBA,研究不同浓度激素及组合对丛生芽诱导、试管苗生根的影响,并进行试管苗练苗和移栽。结果表明:最佳丛生芽诱导培养基为MS+TDZ 0.8mg/L+IBA(0.1～0.5)mg/L,诱导率达到90.0%以上。最佳生根培养基为1/2MS+NAA 0.2mg/L+IBA 0.5mg/L,生根率达到100.0%。勋章菊的叶片是外植体的较好选择,在短期内能获得大量组培苗,移栽成活率也达到95%以上。更多还原

【Abstract】 In vitro seedling leaves of Cazania leaves were used as explants,by using MS as minimal medium,adding different concentration of TDZ,2,4-D,IAA,IBA,effect of different concentration hormone and their combination on the induction of adventitious buds,rooting and transplanting,practice of test-tube seedling were studied.The results indicated that optimum medium for induction adventitious buds was MS+TDZ 0.8 mg/L+IBA(0.1～0.5)mg/L,induction rate of more than 90.0%.The best rooting medium was 1/2MS+IBA 0.5 mg/L+NAA 0.2 mg/L,the rooting rate reached 100%.The leaf explants of Cazania was a better choice,in the short term to obtain large amounts of tissue culture seedlings,the transplanting survival rate could reach more than 95%.更多还原