【摘要】 以五味子叶片和茎段作为外植体进行愈伤组织的诱导,对愈伤组织诱导的影响因素、愈伤组织发育特征以及愈伤组织形成过程中五味子醇甲的积累情况进行研究。结果表明:B5培养基比MS培养基更利于愈伤组织的诱导。根据愈伤组织的发育状态特征可以将其分为4类,其中颜色微黄、质地疏松的C类愈伤组织中所积累的五味子醇甲含量最高。添加1.0mg/L BA、0.2mg/L NAA和0.1mg/L KT的B5培养基上较易诱导出C类愈伤组织,其五味子醇甲含量最高达到4.28mg/g FW。该研究的初步结果对通过愈伤组织悬浮培养生产五味子素的进一步研究奠定了重要基础。更多还原

【Abstract】 Stems and leaf segments of Schisandra chinensis were used as explants for callus induction,and the influencing factors,developed characteristics and the accumulation of Schizandrol A for callus induction were studied.The results showed that B5 medium was effective than MS medium for callus induction.According to the developmentally morphological characters,the formed callus could be classified into four types.C type callus with yellow in color and friability in texture showed the highest accumulation of Schizandrol A.B5medium supplemented with 1.0 mg/L BA,0.2 mg/L NAA and 0.1 mg/L KT were effective to induced C-type callus.Schizandrol A concentration was up to 4.28 mg/g FW in C-type callus.This results was important for further study on large-scale production of schizandrol A by liquid suspension culture of Schisandra chinensis.更多还原