【摘要】 以青海省格尔木蒜脱毒后获得的脱毒小蒜为试材,研究不同培养基、蔗糖浓度、pH值和不同浓度激素配比对脱毒的格尔木大蒜根尖诱导不定芽的影响。结果表明:最佳培养基配比为MS培养基,pH 6.2,蔗糖20 g/L,BA 2 mg/L+2,4-D 0.1 mg/L。pH为6.2时诱导率达81.7%,蔗糖浓度20 g/L时诱导率达55%,激素配比为BA 2 mg/L+2,4-D 0.1 mg/L,诱导率达55%。更多还原

【Abstract】 Golmud small garlic affer virus-free was used as test materials in study on effect of different medium,sucrose concentration,pH,and different concentrations of hormones on induction adventitious bud of root tip from virus-free Golmud garlic.The results showed that the best medium ratio were MS medium,pH 6.2,sucrose 20 g/L,BA 2 mg/L+2,4-D 0.1 mg/L.Induction rate was 81.7% when pH was 6.2,induction rate was 55% when sucrose concentration was 20 g/L,induced rate was 55% when the hormone ratio were BA 2 mg/L+2,4-D 0.1 mg/L.更多还原