【摘要】 本研究对1931~2009年间分离于中国20个省区的167株狂犬病病毒的N基因序列进行进化分析,以探讨中国狂犬病病毒株的基因分型和分组情况、时间和空间的动态进化。结果显示:从中国分离的毒株都属于基因1型狂犬病病毒,可以分为2个进化分支共计8个组,分支Ⅰ包括1~4组,分支Ⅱ包括5~8组;选择压力分析表明中国狂犬病病毒处于较强的净化选择约束下,狂犬病病毒N基因中的核苷酸突变主要是同义突变;运用贝叶斯中的马尔科夫链的蒙特卡洛方法估计中国狂犬病病毒N基因核苷酸的平均碱基替代率为4×10-4替代/位点/年,共同祖先出现的时间不超过2000年前;迁移分析表明中国狂犬病病毒株存在跨地域传播。更多还原

【Abstract】 In order to study phylogeography,population dynamics and molecular evolution of rabies viruses(RABVs) isolates from China,especially spatio-temporal dynamics,the timescale of RABVs evolution and its pattern of migration,we performed an extensive comparative analysis of RABV N gene sequence data,representing 167 isolates sampled from 20 provinces in a 78-year period(from 1931 through 2009).The available Chinese isolates could be divided into two distinct clades:Phylogroup clades I comprised Chinese group 1-4;Phylogroup clades II contained Chinese group 5-8.We found no evidence for positive selection(dN/dS>1) acting at any codon and found strong selective constraints for N gene.Bayesian Markov Chain Monte Carlo(MCMC) analysis suggested that the Chinese rabies viruses originated within the last 2000 years and the mean rates of nucleotide substitution for the N gene were approximately 4×10-4 substitutions per site per year.The analyses of the spatial and spatio-temporal evolution indicated that RABV isolates from China migrated among different Provinces.更多还原