【摘要】 WU多瘤病毒(WUPyV)是多瘤病毒科多瘤病毒属的新成员,近来发现与人呼吸道感染等有关。本研究对2株WUPyV进行全基因组序列测定和拼接,获得这2株病毒全基因组序列,并与已上传到GenBank的国内外几株WUPyV的全基因组序列和氨基酸序列进行比对和系统进化分析。这2株WUPyV是环状、双链DNA病毒,基因组全长5 228bp,比GenBank已知WUPyV全序列少1bp。缺失的一对碱基位于位点4 536处,属于大T抗原的非编码区。病毒全基因组编码5个蛋白,分别是3个衣壳蛋白VP2、VP3、VP1与大T抗原和小T抗原。系统进化分析显示相对中国福建福州的FZ18株,另一株FZTF株与参考株-澳大利亚的B0株关系较近。更多还原

【Abstract】 WU polyomavirus(WUPyV),a new member of the genus Polyomavirus in the family Polyomaviridae,is recently found in patients with respiratory tract infections.In our study,the complete genome of the two WUPyV isolates(FZ18,FZTF) were sequenced and deposited in GenBank(acession nos.FJ890981,FJ890982).The two sequences of the WUPyV isolates in this study varied little from each other.Compared with other complete genome sequences of WUPyV in GenBank(strain B0,S1-S4,CLFF,accession nos.EF444549,EF444550,EF444551,EF444552,EF444553,EU296475 respectively),the sequence length in nucleotides is 5228bp,1bp shorter than the known sequences.The deleted base pair was at nucleotide position 4536 in the non-coding region of large T antigen(LTAg).The genome of the WUPyV encoded for five proteins.They were three capsid proteins: VP2,VP1,VP3 and LTAg,small T antigen(STAg),respectively.To investigate whether these nucleotide sequences had any unique features,we compared the genome sequence of the 2 WUPyV isolates in Fuzhou,China to those documented in the GenBank database by using PHYLIP software version 3.65 and the neighbor-joining method.The 2 WUPyV strains in our study were clustered together.Strain FZTF was more closed to the reference strain B0 of Australian than strain FZ18.更多还原