【摘要】 2006年夏季,本课题组在新疆喀什地区伽师县库蚊标本中分离到我国首株Tahyna病毒XJ0625株,并发现当地不明原因发热患者存在该病毒感染。本研究通过细胞培养、动物实验、电镜观察、间接免疫荧光及交叉保护中和试验等研究对XJ0625病毒株的细胞易感性、动物致病性、形态学及抗原性等特征进行观察,并应用分子生物学软件对其分子进化特征加以分析。结果发现该毒株可以引起BHK-21细胞病变,乳鼠颅内接种该毒株可以引起死亡。与其他布尼亚病毒形态相似,Tahyna病毒为球形有包膜病毒。该病毒与国际流行Tahyna病毒Bardos92株的抗体作用,在间接免疫荧光试验中呈现阳性荧光信号。空斑减少中和试验结果显示该抗体对XJ0625病毒株的中和效价为1∶3 200。核苷酸序列分析显示,该病毒与Bardos92株处于同一个进化分支,二者S节段同源性为91.8%,M节段同源性为81.9%。更多还原

【Abstract】 In 2006,the first Chinese Tahyna virus isolate(XJ0625) was obtained in Xinjiang province and human infection were found in the same region.In this study,cell culture,animal experiments,electron microscopy,immunofluorescence assay and cross neutralization tests were performed to see the cell susceptibility,animal pathogenicity,morphology and antigenic and other biological characteristics of XJ0625.In addition,molecular biology software was used to analyze the characteristics of molecular evolution.The results showed that BHK-21 cell line was susceptible to XJ0625 and the virus was lethal to suckling mice when injected by intracranial ways.Similar to the other Bunyavirus,Tahyna virus is spherical enveloped virus under electron microscopy.XJ0625 infected cells showed strong fluorescent signal and could be neutralied by immune asities fluid with immnity to protype Tahyna virus Bardos 92.The sequence of the S and M segments showed 91.8% and 81.9% homology with Bardos 92.更多还原