【摘要】 目的了解聊城地区2010年手足口病病原情况。方法 2010年6月采集聊城地区240例手足口病病人(包括27例重症病人)咽拭子并提取病毒RNA。采用Real-time RT-PCR方法,以肠道病毒5′UTR区通用引物及探针对240份临床标本进行初步筛查,初筛阳性标本进一步采用CA16及EV71 VP1区特异性引物及探针进行型别鉴定。结果肠道病毒阳性率达70.4%(169/240),其中EV71阳性142例、CA16阳性6例、非CA16和EV71的肠道病毒阳性21例,三者比例约为23.7∶1∶3.5。重症病人中22例为EV71阳性。结论 2010年聊城地区手足口病主要病原为EV71,同时还存在一定比例其他肠道病毒,EV71感染更容易引起重症并发症。更多还原

【Abstract】 Objective To understand the etiology of hand-foot-and-mouth disease in Liaocheng region in year of 2010.Methods Throat swabs from 240 patients with the disease,including 27 severe cases,were collected in Liaocheng in June 2010,and tested for viral RNA by real-time RT-PCR,the samples were initially screened.The positive samples were further appraised by EV71-specific and CA16-specific primer. Results The positive rate of enterovirus was 70.4%(169/240).Of which,142 were EV71 positive,six were CA16 positive,and 21 were non-CA16 and EV71 enterovirus.The ratio among EV71,CA16 and non-CA16 and EV71 was 23.7∶1∶3.5,respectively.Of the 27 severe cases,22 were EV71 positive. Conclusion The main pathogen for hand-foot-and-mouth disease seen in Liaocheng,in 2010,was EV71,and some other enteric viruses were presented at the same time.EV71 infection is much likely to cause severe complications.更多还原