【摘要】 目的探讨PAI-1基因4G/5G多态性与2型糖尿病(2DM)肾病(DN)的相关性,寻找DM的致病基因。方法采用等位基因特异性引物PCR扩增技术检测39例正常人(对照组)和63例2DM病人PAI-1基因4G/5G多态性。结果 DN组PAI-1基因型频率和等位基因频率与对照组比较差异均有显著性(χ2=12.186、7.446,P<0.05),DM组PAI-1基因型频率和等位基因频率与对照组比较差异均有显著性(2χ=24.127、7.312,P<0.05);DN组、DM组4G等位基因频率明显高于对照组,差异有显著性(χ2=6.280,P<0.05)。DN组4G/4G、4G/5G、5G/5G基因型病人尿微量清蛋白排泄率比较差异均有显著性(F=4.922,P<0.05)。结论 4G等位基因存在时,DM的发病风险明显增高,4G等位基因是病人出现清蛋白尿的危险因素。更多还原

【Abstract】 Objective In this study,we investigate the association between the PAI-1 gene 4G/5G polymorphisms with type 2 diabetes with early nephropathy on molecular level,and to explore related virulence gene.Methods The PAI-1 promoter 4G/5G polymorphism in the control group(39 subjects) and the study group(63 patients) were genetyped by allele specific polymerase chain reaction.Results There were significant differences in PAI-1 genetypes frequency and 4G/5G allele frequency between the DN group with the normal group(χ2=12.186,7.446;P<0.05) and between the DM group with the normal group(χ2=24.127,7.312;P<0.05).The DN group and the DM group had a high frequency of 4G allele frequency than that in the normal group(χ2=6.280,P<0.05).In the DN group,the PAI-1 genetype polymorphism was related with the albumin excretion rate(F=4.922,P<0.05).Conclusion The presence of the 4G allele of PAI-1 may involved in the generation of diabetes,the 4G allele of PAI-1 is a risk factor for albuminuria.更多还原