Mouse oocytes were cultured in microgravity simulated by a rotating wall vessel bioreactor and in 1g gravity in vitro,respectively.We examined the fertilization rate of the mouse oocytes matured in different gravity conditions.The fertilization rate of mouse oocytes matured in 1g gravity was 35.82%,and the oocytes matured in simulated microgravity could not be fertilized in vitro.The results suggest that mouse oocytes matura-tion and fertilization ability were compromised by the simulated microgravity in vi...