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改良CTAB法提取鸢尾属植物DNA
DNA Extraction from Iris Species with Modified CTAB Method
【摘要】 鸢尾属植物中酚类、多糖含量较高,DNA提取较为困难。在前人工作基础上,改进了提取植物DNA的CTAB方法,通过延长水浴时间、氯仿/异戊醇(24∶1)2次抽提、适当延长氯仿/异戊醇与提取液接触时间等一系列改进,简化了提取步骤,大大缩短了提取时间。所提取的DNA纯度高,可满足以PCR扩增为基础的试验需要。
【Abstract】 DNA isolation of Iris species is difficult for their high contents of polyphenol and polysaccharide.Here a modified CTAB method for Iris DNA extraction was presented.With series of improvement and simplification of the procedure,including water-bath time extension,chloroform/ isoamyl alcohol(24∶1) twice extraction,high concentration and purity of DNA was obtained and it could be used in the following molecular biology experiments based on PCR amplification.
【基金】 陕西省教育厅项目(2010JK861)
- 【文献出处】 现代农业科技 ,Modern Agricultural Sciences and Technology , 编辑部邮箱 ,2011年08期
- 【分类号】S682.19
- 【被引频次】15
- 【下载频次】644