【摘要】 对中国沿海13种对虾科动物的16S rRNA基因(约371 bp)和细胞色素氧化基因I(COI)部分序列(约385 bp)进行了分析,并采用"最大简约法"、"最小进化法"和"最大似然法"构建了分子系统树。结果表明:在COI基因序列中,有113个位点存在变异(约为总位点数的31.8%),高于16S rRNA基因序列的44个变异位点(约为总位点数的11.9%);构建的分子系统树显示,所构建的NJ、ME和MP树的拓朴结构较为相似,但斑节对虾、日本对虾和缘沟对虾在3个树中相聚位置不同。中国沿海对虾科6属13种,形成3个明显的分支,这3支分别隶属新对虾属、仿对虾属和原对虾属。对虾属、新对虾属、仿对虾属的种间关系与分子系统发育分析结果与传统分类学相一致。16S rRNA序列可能更适用于对虾属以上阶元的遗传多样性分析;COI序列更适合对虾科种间和群体遗传多样性的研究。更多还原

【Abstract】 Partial sequences of 16S rRNA gene(about 371 bp) and cytochrome oxidase I(about 385 bp) gene from 13 shrimp species from South China Sea were analyzed.Molecular phylogenetic trees were reconstructed using Maximum Parsimony(MP) method and Maximum Likelihood(ML) method as well as Maximum Likelihood.According to the results,113 nucleotide sites substituted in COI gene(31.8% of the total nucleotide sites in COI gene)and 44 nucleotide sites substituted in 16S rRNA gene(11.9% nucleotide sites substituted in 16S rRNA gene) were obtained.The phylogeny resulting from the combined 16S and COI partial sequences showed that these species form three well-supported monophyletic clades consistent with the three genera proposed in a recent systematic review of the suborder Dendrobranchiata.This contrasted with conclusions drawn from recent molecular phylogenetic work on penaeid shrimps based on partial sequences of the mitochondrial COI region that support recent revisions of the Dendrobranchiata based on morphological analysis.更多还原