【摘要】 [目的]克隆辽西地区饲养水貂的MLPH基因第一外显子序列及其侧翼序列,预测其结构特征,为培育彩色水貂提供理论依据。[方法]扩增水貂MLPH基因第一外显子和5’侧翼序列,利用SignalP 3.0 Server、ClustalX 1.83、Mega 4.1、TMHM 2.0、ProtComp-v6.0等生物学软件进行序列分析。[结果]测序得到1 029 bp长的序列片段,利用Mega4.1软件计算水貂与大家鼠的遗传距离是最近的0.540 5,与人的遗传距离是最远的0.655 2,构建水貂分子系统进化树。[结论]水貂MLPH基因是一个分泌蛋白。更多还原

【Abstract】 [Objective] To clone exon 1 and its flanking sequences of MLPH gene from minks raised in western Liaoning region and to predict its structure.The study will provide a theoretical basis for breeding of colored mink.[Method] The exon 1 and its 5′-flanking sequences of MLPH gene were amplified from minks.SignalP 3.0 Server,ClustalX 1.83,Mega 4.1,TMHM 2.0,ProtComp-v6.0 and other software were used for sequence analysis.[Result] A 1 029 bp fragment was amplified with the expected size.As calculated with the Mega 4.1,the minks had the nearest genetic distance with rattus(0.540 5) and the farthest with human(0.655 2).Phylogenetic tree of mink was constructed.[Conclusion] The Mink MLPH gene encodes a secretory protein.更多还原