【摘要】 [目的]测定乐清市铁皮石斛总黄酮的含量。[方法]以芦丁为对照品,采用NaNO2-Al(NO3)3-NaOH显色,在510 nm波长处用紫外分光光度法测定总黄酮的含量。[结果]芦丁在0.011 1～0.066 7 mg/ml浓度范围内与吸光度呈良好线性关系(R2=0.999 2);样品溶液在12 h之内稳定性良好,RSD值为0.15%;建立的方法重复性良好,其RSD值为3.12%。[结论]该方法简便、准确、稳定,可用于铁皮石斛中总黄酮含量的测定。更多还原

【Abstract】 [Objective] To determine the content of total flavonoids in Dendrobium officinale Kimura et Migo from Yueqing.[Method] Using rutin as reference substance,contents of total flavonoids in Dendrobium officinale Kimura et Migo from Yueqing were measured at 510 nm by UV spectrophotometry colored with NaNO2-Al(NO3)3-NaOH.[Results] Rutin concentration was linear with absorbance in the range of 0.011 1 to 0.066 7 mg/ml(R2=0.999 2);sample solution was stable within 12 h with RSD value of 0.15%;the established method was good in repeatability with RSD value of 3.12%.[Conclusion] The method was simple,accurate and stable,which could be used to determine the content of total flavonoids in Dendrobium officinale Kimura et Migo.更多还原