【摘要】 [目的]筛选测定乌克兰鳞鲤(Cyprinus carpio)倍性的合适的方法。[方法]建立3种流式细胞术样品制备方法:肾组织单细胞悬液法、红细胞甲醛固定法和红细胞乙醇固定法。采用BD FACS tar Puls型流式细胞仪测定倍性。[结果]3种制样方法均能得到结果,但红细胞甲醛固定法最佳。[结论]流式细胞术可精确、快速地检测乌克兰鳞鲤倍性,适应其多倍体产业化的需要。更多还原

【Abstract】 [Objective]The aim was to screen the suitable determination method of the ploidy of Ukraine Carp.[Method]Three methods of sample treatment for Flow Cytometry were developed to determine the ploidy of Ukraine Carp,including unicellular system from renal tissues,red cells fixed with formaldehyde and red cells fixed with ethanol.The ploid of samples were determined by FCM of BD FACS tar Puls.[Result] It proved that these methods could all get result and the method red cells fixed with formaldehyde were the best.[Conclusion]To accommodate the industrialization of polyploid,flow cytometry is the best way to examine fish ploidy with merits of high definition and speediness.更多还原