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通过酵母双杂交筛选水稻类受体激酶CR4的胞外结合蛋白
Screening of Extracellular Binding Proteins of Rice Receptor-like Kinase CR4 by the Yeast Two-hybrid
【摘要】 [目的]寻找CR4的胞外结合蛋白。[方法]采用OsCR4的胞外域作为诱饵蛋白,酵母双杂交筛选14 d的小苗cDNA文库。[结果]得到了许多蛋白,包括一个过氧化物B(D26484)、甲硫氨酸硫氧还原酶(ABF96078)和一个未知功能的蛋白。[结论]为研究CR4的信号转导机制提供了理论依据。
【Abstract】 [Objective] The research aimed to find the extracellular binding proteins of CR4.[Method] The extracellular region of OsCR4 was as the bait protein,and the yeast two-hybrid was used to screen the seedling cDNA library which was cultivated 14 d.[Result] A lot of proteins which included a peroxid B(D26484),a methionine thioredoxin reductase(ABF96078) and an unknown function protein were gained.[Conclusion]It provided the theory basis for studying the signal transduction mechanism of CR4.
【关键词】 水稻;
类受体激酶;
胞外结合蛋白;
酵母双杂交;
【Key words】 Rice; Receptor-like kinase; Extracellular binding protein; Yeast two-hybrid;
【Key words】 Rice; Receptor-like kinase; Extracellular binding protein; Yeast two-hybrid;
【基金】 2010年石家庄市科学技术研究与发展项目(10120803);2007年石家庄学院科研启动基金立项项目(2007012);2008年度石家庄学院教学改革研究项目(2008006)
- 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2011年08期
- 【分类号】Q78
- 【被引频次】2
- 【下载频次】170