【摘要】 [目的]探索中微量元素对黄瓜黑星病的防治效果及其对黄瓜植株酶活性的影响。[方法]通过室内抑菌试验及盆栽试验,研究不同中微量元素对黄瓜黑星病的防治效果;同时检测喷施中微量元素化合物后7 d黄瓜苗期叶片的可溶性蛋白、丙二醛含量,及过氧化物酶(POD)和超氧化物歧化酶(SOD)的活性。[结果]3种微量元素对黑星病菌均有一定抑制作用,以氯化钙处理效果最为显著,防效达到66.9%;硼酸和硫酸锌处理的防效分别为54.6%和24.1%。黄瓜植株遭受黑星病原菌侵害后,几种不同中微量元素的施用均能有效降低黄瓜叶片中MDA含量,锌和硼处理能显著提高黄瓜植株体内蛋白质含量,锌和钙处理均可诱导黄瓜植株中POD和SOD活性上升,从而通过减缓细胞膜质的受损程度来提高黄瓜植株对黑星病菌的抵抗能力。[结论]该研究结果为中微量元素防治黄瓜黑星病的实际应用及其抗病机理研究提供了理论基础。更多还原

【Abstract】 [Objective]The control effect of trace and secondary elements on scab of cucurbits and their effects on enzyme activities of cucumber plants were explored.[Method]Indoor antifungal experiment and pot test was carried out to study the control effect of different trace and secondary elements on scab of cucurbits;after trace and secondary element compounds were sprayed for 7 d,soluble protein,malondialdehyde,peroxidase and SOD activity of cucumber leaves during seedling stage was simultaneously detected.[Result]Three trace elements all had certain inhibition effects on scab of cucurbits,the effect of calcium chloride treatment was the most significant,while the control efficiency reached 66.9%;the control effect of boric acid and zinc sulfate treatment were 54.6% and 24.1%.When cucumber plants were infected by scab of cucurbits,the application of different trace and secondary elements all could effectively reduce MDA content in cucumber leaves,zinc and boron treatment could significantly improve the protein content in cucumber plants,zinc and calcium treatment could induce POD and SOD activity in cucumber plants increase,thereby slowing the damage level of the cell membrane to improve the resistance of cucumber plants against scab of cucurbits.[Conclusion]The results provided the theoretical basis for practical application and research on resistance mechanism of using trace and secondary elements to control scab of cucurbits.更多还原