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南山茶茶籽多酚的提取及测定
Extraction and Determination of Polyphenols from Camellia Semiserrata Seed Cake
【摘要】 [目的]研究南山茶籽饼粕中多酚类物质的提取及测定。[方法]采用L9(34)正交试验设计,研究了不同的料液比、提取时间、乙醇浓度和提取温度对多酚提取率的影响。[结果]最优浸提南山茶籽饼粕多酚工艺条件为:40%乙醇浓度、提取时间2h、提取温度65℃、料液比1∶25。该条件下的多酚含量达到了3.5%,对植物病原真菌(炭疽菌)菌丝生长具有明显的抑制作用。[结论]该研究为南山茶籽饼粕的开发利用提供了理论依据。
【Abstract】 [Objective]The aim was to study the extraction and determination of polyphenols in Camellia semiserrata seed cake.[Method]The orthogonal experimental design method L9(34) was adopted to study the impacts of different solid-liquid ratio,extraction time,alcohol concentration and extraction temperature on extraction rate of polyphenols.[Result]The results showed thai the optimum extraction conditions were as follows: 40% ethanol concentration,extraction time 2 h,extraction temperature 65 ℃,solid-liquid ratio 1∶25 and the content of polyphenol reached 3.5% and the polyphenols of Camellia semiserrata seed cake significantly inhibited the growth of plant pathogenic anthracnose hypha.
【Key words】 Camellia semiserrata seed cake; Polyphenols; Orthogonal design; Inbibitional effect;
- 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2011年01期
- 【分类号】S685.14
- 【被引频次】4
- 【下载频次】273