【摘要】 目的:评价一次性筷子、牙签及纸杯浸提液对小鼠L-929细胞的毒性作用。方法:参照国家标准,制备一次性筷子、牙签及纸杯等样品浸提液,采用样品浸提液配制培养液培养小鼠L-929细胞,在光镜下观察细胞生长形态,采用细胞计数法计算细胞失贴壁率,采用WST-1法测定细胞活性并计算细胞增殖度。结果:与对照组相比,采用浸提液配制的细胞培养液培养细胞4d或7d后,各组细胞出现凋亡或坏死征象,细胞失贴壁率明显升高(P<0.05),细胞活性及细胞增殖度明显下降(P<0.05)。结论:一次性筷子、牙签及纸杯用具等能影响L-929细胞生长及增殖,可能具有潜在的毒性作用。更多还原

【Abstract】 OBJECTIVE:To evaluate the toxic effects of the extracted solution of disposable chopstick, toothpick or cup in murine L-929 cells.METHODS:According to the national standards,we prepared cell culture medium with the extracted solution of disposable chopsticks,toothpicks or cups.The cell morphology of murine L-929 cells was examined microscopically and cell viability was detected with WST-1 staining method.The detached cell ratios and the relative growth rate of L-929 cells were calculated.RESULTS:Compared with the control,after treatment for 4 days or 7 days,cells in the experimental groups showed signs of apoptosis or necrosis.The detached cell ratios were increased significantly(P<0.05),while cell viability and relative growth rate declined significantly(P<0.05). CONCLUSION:Treatments with extracted solution of disposable chopstick,toothpick or cup could affect cell growth and proliferation,thus probably possess potential toxic effects.更多还原