【摘要】 目的:探讨锰超氧化物歧化酶(manganese superoxide dismutase,MnSOD)基因单核苷酸多态性与食管癌放疗敏感性的关系。方法:以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法分析93例食管癌患者外周血白细胞DNA的MnSOD基因第16密码子多态性(Ala16Valrs4880);通过比较食管癌放疗的局部疗效,分析携带MnSOD基因野生型(T/T)与突变基因型(T/C、C/C)食管癌患者对放疗的敏感性差异。结果:放疗后局部疗效达完全缓解者(CR)者22例、部分缓解者(PR)者63例,无缓解(NR)者8例,其MnSOD基因第16密码子多态性(T/T、T/C+C/C)分布频率分别为59.1%、40.9%;77.8%、22.2%;62.5%、37.5%,3者之间差异无统计学意(x~2=3.223,P>O.05)。在食管癌病变长度≤5cm的患者中CR与PR、NR之间MnSOD(T/T、F/C+C/C)基因型分布频率的差异无统计学意义(x~2=2.658,P>0.05);而病变长度>5cm的患者中携带C等位基因患者的疗效优于携带T/T基因型患者。MnSOD(T/T、T/C+C/C)基因型患者的中位生存时间和1年生存率分别为17个月和68%;22个月和73%,但T/T与T/C+C/C基因型之间的差异无统计学意义(P>0.05)。结论:病变长度>5 cm患者中,携带T/C或C/C基因型患者放疗对肿瘤的治疗效果优于携带T/T基因型患者。更多还原

【Abstract】 OBJECTIVE:To investigate the association of single nucleotide polymorphisms of(manganese superoxide dismutase,MnSOD) gene with radiosensitivity of esophageal squamous cell carcinoma.METHODS:The MnSOD Ala16Val was genotyped by polymerase-chain reaction-restriction fragment length polymorphism(PCR-RFLP) analysis in 93 esophageal squamous cell carcinoma patients.All patients received 3D-CRT or the conventional radiotherapy.RESULTS:The frequencies of T/T and C/T+C/C allelotype among three grades of curative effect were 59.1%,40.9%;77.8%,22.2%;62.5%,37.5%respectively.There was no statistical difference in allelotype distribution between three grades.When the length of esophagus cancer was 5 cm or less than there was no statistical difference in allelotype distribution between three grades of curative effect.But when the length was more than 5 cm a significant difference was observed in the MnSOD allelotype distribution among different length(x~2=2.658,P<0.05).The curative effect of radiotherapy in patients with C/T+C/C allelotype was better than patients with allelotype T/T when the length was more than 5 cm.There was no difference of MST and 1-year survival rates in patients with MnSOD-9 T/T genotype and MnSOD-9 T/C + C/C genotype(17 months,68%,35.82%vs 22 months,73%,34.62%P>0.05).CONCLUSION:The MnSOD Ala16Val appears to be associated with an improved radiosensitivity in patients when the length of esophagus lesion was more than 5 cm.更多还原