【摘要】 目的探讨脑出血(ICH)后激活腺苷A2A受体对中性粒细胞浸润、TNF-α蛋白的表达及细胞凋亡的影响。方法 SD大鼠随机分为对照组、ICH组、CGS组、CGS+ZM组、ZM组。应用胶原酶脑内立体定向注射制作ICH模型。对照组注射生理盐水2ul,CGS组、CGS+ZM组、ZM组在ICH模型制作前15min,脑内立体定向分别注射CGS1μg,CGS1μg+ZM1μg,ZM1μg。模型制作后24h处死动物,应用HE染色检测中性粒细胞的浸润,应用Western-Blot技术检测TNF-α蛋白的表达,应用TUNEL法检测细胞凋亡。结果 ICH组可见明显的中性粒细胞浸润、TNF-α蛋白的表达及凋亡的细胞,与对照组比较,差别具有显著性(P<0.001)。CGS组中性粒细胞浸润、TNF-α的表达及凋亡的细胞数目明显降低,与ICH组比较,差别具有显著性(P<0.001)。CGS+ZM组中性粒细胞浸润、TNF-α的表达及凋亡的细胞明显增高,与ICH组比较差异无显著性(P>0.05),与CGS组比较差异具有显著性(P<0.001)。结论选择性的腺苷A2a受体激活可降低ICH后的炎症反应和细胞凋亡,从而减轻ICH后组织的损伤。更多还原

【Abstract】 Objective To investigate the effect of adenosine receptor A2a activation on the neurophil cellular infiltration,expression of TNF-α protein and apoptosis after intracerebral hemorrhage(ICH).Methods SD rats were randomly divided into control,ICH,CGS,CGS+ZM,and ZM groups.ICH models were performed by collagenase stereotaxical injection.Control group were injected 2 ul physiologic saline.CGS,CGS+ZM and ZM group were respectively injected CGS 1 μg,CGS 1 μg+ZM 1 μg and ZM 1 μg at 15 minutes before ICH models were performed.Rats were put to death at 24 th hour after modeling.Neurophil cellular infiltration was detected by HE dyeing.Expression of TNF-α protein was determined by Western-blot method.Apoptosis was measured by TUNEL method.Results Neurophil cellular infiltration,expression of TNF-α protein and apoptosis in ICH group were increased and had significant difference compared with control group(P<0.001).Neurophil cellular infiltration,expression of TNF-α protein and apoptosis in CGS group were decreased and had significant difference compared with ICH group(P<0.01).Neurophil cellular infiltration,expression of TNF-α protein and apoptosis in CGS+ZM group were incereased and had no significant difference compared with ICH group(P>0.05).Conclusions Selective adenosine receptor A2a activation can depress inflammation and apoptosis after cerebral hemorrhage,and might reduce tissue injury in or surround hemotoma.更多还原