【摘要】 目的:研究11-羰基-β-乳香酸的抗癌作用,并探讨其作用机制。方法:用MTT法和生长曲线法检测11-羰基-β-乳香酸对人结肠癌HCT-116细胞增殖的抑制作用,计算半数有效抑制浓度(IC50)值;用Hoechst33258荧光染色法观察经药物处理后细胞核的形态变化;用流式细胞仪测定药物处理后HCT-116的细胞周期和细胞凋亡率。结果:结果提示11-羰基-β-乳香酸浓度为15～50μg/ml时,对HCT-116细胞的48h抑制率为8.7%～90.16%,48h的IC50为27.36±0.14μg/ml;生长曲线结果提示11-羰基-β-乳香酸对HCT-116细胞生长的抑制作用呈明显的时效和量效关系;30μg/ml的药物作用48h后,Hoechst33258染色结果显示细胞核出现细胞核固缩,染色质不均一或边集呈致密强荧光,凋亡小体等明显的凋亡形态学变化;流式仪检测结果提示11-羰基-β-乳香酸可阻滞细胞周期并诱导细胞凋亡。结论:11-羰基-β-乳香酸在体外对结肠癌具有较强的抗癌作用,能够阻断HCT-116细胞周期,诱导细胞凋亡。更多还原

【Abstract】 Objective:To investigate antitumor effects of 11-carbonyl-β-boswellic acid. Methods:MTT assay and growth curve assay were used to assess the inhibitory effect of 11-carbonyl-β-boswellic acid,the cell cycle and the cellular apoptosis were measured using flow cytometry. Results:The proliferation of HCT-116 was obviously inhibited in dose-dependent manner. 11-carbonyl-β-boswellic acid can block cell cycle.Annexin V/PI double staining showed that the apoptosis rate increased by HCT-116 cells induced by 11-carbonyl-β-boswellic acid 48h later. Conclusion:11-carbonyl-β-boswellic acid can inhibit the proliferation of colon tumor cell,induce HCT-116 cells to apoptosis and block their cell cycle.更多还原