【摘要】 目的了解α1-抗糜蛋白酶(AACT)基因突变与昆明儿童哮喘发生是否具有相关性。方法应用聚合酶链反应(PCR)技术、限制性片段长度多态性(RFLP)分析方法,选择昆明市汉族哮喘儿童及正常儿童各50例,对α1-抗糜蛋白酶基因外显子(exon)Ⅱ、Ⅲ进行检测。结果所有研究对象均未发现α1-抗糜蛋白酶基因Bochum-1、Bonn-1突变型。结论α1-抗糜蛋白酶基因Bochum-1、Bonn-1突变型与50例昆明市汉族儿童哮喘发病无相关关系。更多还原

【Abstract】 Objective:To understand α1-antichymotrypsin gene mutations of children asthma in Kunming.Methods:50 patients with children asthma and 50 controls were tested for exon Ⅱ,Ⅲ of α1-antichymotrypsin by PCR,RFLP to analyze AACT encoding gene.Results:Bochum-1,Bonn-1 mutations of the AACT gene were not found in any of the subjects.Conclusion:There is no relationship between α1-antichymotrypsin gene mutations including Bochum-1,Bonn-1 and 50 han asthmatic children in Kunming.更多还原