【摘要】 目的建立较为简便的新生乳鼠海马神经细胞原代培养方法。方法急性分离新生24h内的大鼠乳鼠海马组织,用胰蛋白酶消化与机械吹打相结合的方法分离海马神经细胞,以含10%胎牛血清的培养基种植、无血清饲养液维持饲养,神经微管相关蛋白2(Map-2)鉴定神经元纯度。结果培养8d,神经元胞体清晰,结构完整,神经元纯度最高达到85.71%。结论该法无需严格控制消化时间,也不使用阿糖胞苷纯化细胞,即能够获得生长状态良好、纯度较高的神经元。更多还原

【Abstract】 Objective To establish a simple method for the primary culture of hippocampal neurons of newborn rats.Methods Hippocampal neurons of rats born within 24 hours were isolated by the method of trypsin digestion combining with mechanical dispersion,then were planted in the medium containing 10 % fetal bovine serum,and were given maintenance feeding with serum-free medium.Identification of neurons and its purity were conducted by neural microtubule-associated protein(Map-2)method.Results The structures of nerve cells were clear and integral,and the purity of the neurons was up to 85.71 % after culturing for 8 days.Conclusion Unnecessary to strictly control the digestion time or to use the Ara-C for purifying the cells,neurons with good condition and high purity can be obtained by the established method.更多还原